lucigen MTE0970 TE Buffer

　　產(chǎn)品簡介:北京中北林格供應(yīng)lucigen MTE0970 TE Buffer，中北林格是lucigen總代理，提供lucigen產(chǎn)品貨號報價查詢。其他品牌包括cellscript總代理，Biosearch technologies總代理，epicentre總代理，illumina總代理，ICLLAB總代理，ImmunoReagents總代理。</p>

　　lucigen MTE0970 TE Buffer產(chǎn)品參數(shù)

　　產(chǎn)品名稱:TE Buffer</p>

　　貨號:lucigen MTE0970</p>

　　規(guī)格:70 ml</p>

　　存儲溫度:+4℃</p>

　　品牌:lucigen</p>

　　供應(yīng)商:中北林格</p>

　　產(chǎn)地:us</p>

　　發(fā)貨地:北京</p>

　　lucigen MTE0970 TE Buffer 產(chǎn)品詳情

　　Applications

　　Purification of TNA, DNA, or RNA for many applications, including PCR, RT-PCR, cloning, sequencing, Northern and Southern blotting, restriction analysis, and genomic library preparation.

　　The MasterPure™ Complete DNA and RNA Purification Kit uses a novel technology that enables efficient purification of intact total nucleic acid (TNA), DNA, or RNA from every type of biological material (Table 1 and Fig. 1).

　　The gentle salt-precipitation protocol permits rapid purification of high-molecular-weight nucleic acids from a single sample or many samples that are processed simultaneously (Fig. 4). High yields of nucleic acids are obtained using MasterPure Kits (Table 2), and yields and purity are highly consistent for different samples of the same material (Fig. 2). The resultant nucleic acids have an A260/A280ratio of 1.8-2.0, indicating that they are substantially free of protein. The TNA, DNA, and/or RNA purified from different samples can be used directly in many applications. For example, the sensitivity of PCR or RT-PCR assays is often improved if the template is obtained using a MasterPure Purification Kit.

　　應(yīng)用

　　用于許多應(yīng)用的TNA，DNA或RNA的純化，包括PCR，RT-PCR，克隆，測序，Northern和Southern印跡，限制性分析和基因組文庫制備。

　　MasterPure™完整DNA和RNA純化試劑盒采用了一種新技術(shù)，可以從各種生物材料中有效純化完整的總核酸（TNA），DNA或RNA（表1和圖1）。

　　溫和的鹽沉淀方案允許從單個樣品或同時處理的許多樣品中快速純化高分子量核酸（圖4）。使用MasterPure試劑盒獲得高產(chǎn)量的核酸（表2），并且對于相同材料的不同樣品，產(chǎn)率和純度高度一致（圖2）。得到的核酸的A260/ A280比率為1.8-2.0，表明它們基本上不含蛋白質(zhì)。從不同樣品中純化的TNA，DNA和/或RNA可以直接用于許多應(yīng)用中。例如，如果使用MasterPure純化試劑盒獲得模板，則PCR或RT-PCR測定的靈敏度通常會提高。

　　Benefits

　　Purify TNA in 30 minutes without cumbersome columns.

　　A260/A280ratios consistently between 1.8 and 2.0.

　　Recover >90% of theoretical DNA yield.

　　No phenol, chloroform, or other caustic solvents.

　　Each kit component is also available separately in a 10X package size.

　　Low- and high-molecular-weight nucleic acids are recovered.

　　優(yōu)點

　　在30分鐘內(nèi)純化TNA，無需繁瑣的色譜柱。

　　阿260/ A280個比例一致1.8和2.0之間。

　　恢復(fù)> 90％的理論DNA產(chǎn)量。

　　不含酚，氯仿或其他腐蝕性溶劑。

　　每個套件組件也可單獨提供，封裝尺寸為10X。

　　回收低分子量和高分子量核酸。

　　lucigen MTE0970 TE Buffer圖示說明

　　Figure 1 (click to enlarge). Overview of the MasterPure™ Complete Kit protocol.

　　圖1（點擊放大）MasterPure™完整套件協(xié)議概述。

　　Figure 2. Comparison of PCR sensitivity using templates obtained using the MasterPure™ Complete DNA and RNA Purification Kit versus a spin-column kit. PCR amplification was performed after extraction from serial dilutions of E. coli cells ranging from 2 x 107 (lanes 1) to 200 (lanes 6). Lane M, DNA marker; lane 7, negative control.

　　圖2.使用MasterPure™完整DNA和RNA純化試劑盒與旋轉(zhuǎn)柱試劑盒獲得的模板比較PCR靈敏度。從大腸桿菌細(xì)胞的系列稀釋液中提取后進(jìn)行PCR擴(kuò)增，范圍為2×107（泳道1）至200（泳道6）。泳道M，DNA標(biāo)記;第7道，陰性對照。

　　Figure 3 (click to enlarge). DNA, RNA, and total nucleic acid (TNA) purified from diverse cell sources using the MasterPure™ Complete Kit. M = kb ladder.

　　圖3（點擊放大）使用MasterPure™完整試劑盒從不同細(xì)胞來源純化DNA，RNA和總核酸（TNA）。M = kb階梯。

　　Figure 4. Consistent purification of DNA, TNA, and RNA. Five different genomic DNA (lanes 1-5), TNA (lanes 6-10), and total cellular RNA (lanes 11-15) samples were isolated from frozen bovine liver with the MasterPure™ Complete DNA and RNA Purification Kit. Lane M, Kilobase

　　圖4. DNA，TNA和RNA的一致純化。用MasterPure TM Complete DNA和RNA純化試劑盒從冷凍牛肝中分離5種不同的基因組DNA（泳道1-5），TNA（泳道6-10）和總細(xì)胞RNA（泳道11-15）樣品。Lane M，Kilobase

　　Table 1. Examples of targets and samples analyzed using MasterPure™ DNA and RNA Purification Kits.Using an extremely simple salt-precipitation protocol, TNA, DNA, or RNA can be isolated from virtually any source in less than 1 hour.

　　*For extraction of yeast DNA, the MasterPure™ Yeast DNA Purification Kit is recommended.

　　Table 2. Example of yields of nucleic acids obtained using MasterPure™ Complete DNA and RNA Purification Kits.

　　對此產(chǎn)品有興趣，可聯(lián)系中北林格獲取更多詳細(xì)信息。</p>